Background:

The blue fluorescent protein (BFP) is a mutant of the green fluorescent protein, where the phenolic ring of the chromophore has been replaced by imidazole cycle of histidine residue. The usability of BFP as a fluorescent marker is hampered by its low fluorescence quantum yield at room temperature. The intensity of fluorescence increases by a factor of 4.5 when the temperature is decreased from 320 K down to 225 K. The fluorescence is also enhanced by hydrostatic pressure. Both effects have been explained by shift of the equilibrium between hydrogen nonbonded and hydrogen-bonded chromophores.

Applications:

WB

Name of antibody:

mBFP

Immunogen:

This antibody is raised against a recombinant protein and recognizes mBFP.

Full name:

monomeric blue fluorescence protein

SwissProt:

P80893

WB Predicted band size:

26 kDa

WB Positive control:

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mBFP ne) cDNA for 48 hrs and lysed

WB Recommended dilution:

1000-5000